Bd facscanto ii is a flow cytometer intended for the qualitative and quantitative measurement of biological and physical properties of cells and other particles to generate multiparametric results for in vitro diagnostic use. It is equipped with 3 low power lasers, at wavelengths of 488 nm blue, 633 nm red and 405 nm violet. Bd biosciences is approved as a provider of continuing education programs in the clinical laboratory sciences by the ascls p. Bd facscanto clinical software includes specific application modules optimized for use with specific ivd reagent kits. With optimal reproducibility, the bd facscanto ii system reduces handson technician time and costs associated with repeat testing. Bd facscanto ii quick reference manual pdf download. Bd biosciences facscanto ii flow cytometer features. It includes automated setup and compensation, applicationspecific analysis modules, and predefined reports with integrated quality control. Interference there are no known incompatibilities with bd facscanto clinical. Jan 25, 2017 briefly, floating and attached cells were collected at 24, 48 and 72 h after bosutinib treatment at 1.
Additionally, when used with bd trucount standard tubes, the. Using bd panels with bd facs spa and bd facscanto clinical software and the bd facslink lis interface. Sep 26, 2018 measurements were performed immediately on a facscanto ii bd biosciences. Bd facscanto ii features bd facscanto ii clinical cell analyzer. Instrument installation, operational, and perf clinical cytometry. Our bd facscanto ii is a 3 laser red 633nm, blue 488nm and violet 405nm cytometer capable of analyzing up to 8 fluorescence parameters and 2 scatter parameters fsc and ssc. A bd hlab27 software module for the bd facscanto ii system provides a complete system for rapid detection of hlab27 antigen, which is clinically relevant to the evaluation of seronegative spondyloarthropathies. For use only with bd facs 7color setup beads for setup and reagents. Sections are indicated with the appropriate software icons. Volumetric aquios cl and beadbased facs canto ii cytometers january 2017 cytometry part b clinical cytometry 94rr2. The bd facscanto ii is designed to address the needs of todays busy clinical lab. Other software fcs and setup files created within bd facscanto clinical software can be imported into bd facsdiva software v5. Bd facscanto fluidics with bd facscanto clinical software or bd facsdiva software.
Bd facsdiva software pc performs sample acquisition 18 bits5 decades and data analysis. Numerous innovations make the system easy to use, powerful, and productive. Issuu is a digital publishing platform that makes it simple to publish magazines, catalogs, newspapers, books, and more online. Ifnbeta is a safe and effective drug for the treatment of rrms 1,2. Instruments available for use at the flow cytometry core facility, institute of cell and molecular science, barts and the london, queen mary, university of london. Running samples with bd facscanto clinical software 89. Samples were analyzed on the bd facslyric system with bd facsuite clinical software, and on the bd facscanto ii system with bd facscanto clinical software and bd facs 7color setup beads. The bd bioscience facscanto ii flow cytometer is a benchtop analyzer providing reliable and accurate results for experiments with up to 10 parameters. These tools assist in the ease and efficiency of using facscanto as they enable automated features. Bd facscanto ii flow cytometer reference manual alfresco. Follow only those sections that apply to the software you are running. Selecting reagents for multicolor flow cytometry with bd lsr. Mar 10, 2017 using a predefined gating strategy, average frequencies technical replicates divided by 2 for each parameter were compared between the three flow cytometers bd facscanto tm ii, bd lsrfortessa.
The february 2020 security rollupcumulative update was released in february 2020. Bd compbeads compensation particle sets are designed to optimize compensation settings in multicolor flow cytometry. Refer to the reagent package insert for applicationspecific limitations. We partner with leading nonprofit organizations and foundations to address unmet healthcare needs, and we promote the use of best practices and applied research when it comes to tackling healthcares biggest challenges. Bd facscanto ii flow cytometry system becton dickinson. The bd facscanto ii provides the ultimate in flexibility and can be configured with two or three lasers to. System options include 8color 3laser system 405 488 633 nm 8color 2laser system 488 633 nm a 2d barcode reader option a choice of two automated sample loading systems with integrated covers. Standardized and flexible eight colour flow cytometry panels. The optics of the bd facscanto ii system consist of an excitation source with up to three lasers. Jan 21, 2012 facscanto ii offers walkaway sample handling with the carousel loader 40 tubes capacity or high throughput sampler 96 or 384well plate loader. Before you begin z stain either the high or the low bd stem cell control.
Our pc2 facility currently houses one bd facscanto tm ii analyser 3laser, 8color and one bd facsaria tm iii sorter 4laser, 12color. Implementation of the ogata flow cytometric scoring system in. Maximize buttonfills the screen with the main window. Since response to this treatment is heterogeneous in ms, searching for biomarkers that allow early identification of patients with high probability of showing an optimal response is of great clinical importance.
Bd facscanto ii flow cytometer quick reference guide this guide contains instructions for both bd facscanto clinical and bd facsdiva software. Development of a novel anticd19 chimeric antigen receptor. Bd biosciences bd facscanto ii flow cytometers 422 and 53 configurations with bd facscanto clinical software and bd facsdiva software. Concurrently, we describe the implementation of our car t cell production system, using lentiviral vector and clinimacs prodigy, within a mediumsized academic institution. This webcast introduces endusers to bd iv fluids and discuss productprocedure details that will help ensure a. Facscanto ii system is majorly comprised of a flow cytometer, a fluidics cart, and computer workstation. One striking difference between the facscanto and older generation machines is that this system operates on a pcbased windows xp platform using bd facsdiva software. Find atlascopco, koch, nikon, and olympus for sale on machinio. Vitro diagnostics assays including bd s tbnk analysis kits.
View online or download bd facscanto ii instructions for use manual, quick reference manual. The end result is a patented optical design for enhanced signal retention coupled with digital electronics that capture up to 10,000 events per second. Bd facscanto ii is a flow cytometer intended for the qualitative and. Bd facscanto ii measuring instruments quick reference. Bd biosciences facscanto ii flow cytometer features optics.
Jun 07, 2006 the bd facscanto is often hailed as the new generation of flow cytometry. This video provides testimonials from hospitals and health systems using bd healthsight analytics and practice improvement services share their goals and outcomes. Bd facscanto ii is a 3laser 9color flow cytometry analyzer provided by becton dickinson bd. Bd facs 7 color setup beads are for in vitro diagnostic use on bd facscanto or bd facscanto ii systems. Minimize, maximize, and close buttons in title bar minimize buttonreduces the application to a button on the windows taskbar. Saves time, improves reproducibility high performance, innovative system the bd facscanto ii fluidics cart is designed to save time and improve system performance. Win32k patch was released with december 2019 security updates, crypto api patch was released with january 2020 security updates. Bd facs canto ii flow cytometry system gmi trusted. Bd bioscience facscanto ii flow cytometer marshall scientific. Bd facscanto ii resources cell analyzer research instrument. Cytometry bd facscanto ii laser errors happy new year to all.
Accurate and reproducible enumeration of t, b, and nk. Standardization of 8color flow cytometry across different flow cytometer instruments. Data files for the included patients were reanalyzed using facsdiva 8. Bd facscanto clinical software is supplied with the bd facscanto and facscanto ii systems and includes application panels optimized for use with specific ivd reagent kits. Page 1 bd facscanto ii flow cytometer quick reference guide this guide contains instructions for both bd facscanto clinical and bd facsdiva software.
Bd facscanto clinical software can import worklists from bd facs sample prep assistant spa software v2. Home products manufacturers bd biosciences bd facscanto ii products. The beads are run with bd facscanto clinical software. Selecting reagents for multicolor flow cytometry with bd lsr ii and bd facscanto systems article in nature methods 512 december 2008 with 81 reads how we measure reads. As a leading provider of tools for flow cytometry, bd biosciences is committed to offering comprehensive, innovative software. Laser excitation optics illuminate cells in the sample and collection optics direct light scatter. Bd facscanto ii becton dickinson, san jose, ca, usa. These include gating tools to automatically find a population, biexponential scaling to completely see populations that might have negative relative fluorescence intensities, and even overlays to compare multiple tubes or samples. Apart from the diva software, the bd facscanto ii system includes a clinical software designed to address the needs of todays busy clinical laboratory.
Provides streamlined software workflows for acquisition and analysis. Page 60 run setup again optimize setup values proceed to optimizing with using bd facscanto bd facscanto clinical when prompted, clinical software software on page 64. Bd facscanto ii flow cytometer analyser sahmri research. A feasibility study in clinical laboratories in switzerland. The ivd cleared bd facscanto ii system includes software designed to address.
Bd facscanto clinical software with spa ii sample prepper bd facscanto clinical software with spa iii sample prepper using bd panels with bd facscanto clinical software and the bd facslink lis interface. Multicentre validation of a flow cytometry method to. The bd facscanto ii system can optionally be used with the bd facs loader for automatic sample introduction, a standalone barcode reader for data input into bd facscanto clinical software, and with the bd facs sample prep assistant ii for automatic sample preparation of. Strengthening health systems and expanding access to care. View online quick reference manual for bd facscanto ii measuring instruments or simply click download button to examine the bd facscanto ii guidelines offline on your desktop or laptop computer. At the heart of the cytometer, for example, the fluidics system features a fixedalignment flow cell to minimize startup time and improve reproducibility. The current study was performed by reanalysis of retrospective data.
Apoptosis detection was performed using bd facscanto ii flow cytometer and data was analyzed using the bd facsdiva software bd biosciences, san jose, ca, usa. Implementing standard operating procedures sop for. All other cytometer and fluidics cart functions are controlled by bd facscanto clinical software and bd facsdiva software provided with the instrument. Somflow offers comprehensive training and education, experimental design and protocol guidance specifically targeting effective data generation and interpretation in the field of flow cytometry. Bd facs canto ii operators manual marshall scientific issuu. These panels feature automated gating, calculations, and report generation to deliver consistent, reproducible, and standardized analysis. It automatically sets cytometer detector voltages and creates compensation tables based on a single run with bd facs 7color setup beads.
The bd facscanto ii fluidics system is designed to streamline work, save time, and improve performance. Bd facscanto clinical software set up the cytometer with bd facscanto clinical software, even if you. A fixed alignment flow cell, for example, minimizes startup time and improves reproducibility. Bd facscanto fluidics with bd facscanto clinical software. This system provides application specific setup, including automated compensation and. Our software is designed to address the needs of todays busy clinical. The bd facscanto clinical software is used for fda. Bd bioscience facscanto ii 3 laser flow cytometer used. At a time when health care systems around the globe are struggling to keep up with the influx of patients from the covid19 crisis, bd employees with clinical backgrounds are stepping up to help. Bd facscanto clinical software contains modules for dedicated clinical applications with automatic gating algorithms, while bd facsdiva software is nonapplication specific. It delivers productivity, consistency, and reliability with features that include automated setup and compensation using bd facs 7color setup beads, applicationspecific analysis.
Bd facscanto ii becton dickinson, san jose, ca, usa data file structure. Multisite evaluation of the bd stem cell enumeration kit for. The bd facscanto family of benchtop analyzers features reliable performance and accurate results for experiments requiring up to 10 parameters. Bd facscanto flow cytometer 433 configuration with bd facscanto clinical software and bd facsdiva software and bd facscanto ii flow.
Close buttonexits the application and prompts the fluidics shutdown procedure. Becton dickinson bd facscanto ii and beckman coulter bc navios, aiming at increasing reproducibility and interlaboratory comparability of immunophenotypic data in clinical laboratories in. Bd facsdiva software provides a wide array of basic analysis features, enabling multiple applications to run on the bd facscanto, bd lsr, and bd facsaria cytometers. Flow cytometry analyser ivd, automated a mains electricity acpowered laboratory instrument intended to be used to count, examine andor sort cells or microscopic particles in a clinical specimen e. Bd facscanto ii 3 bd facscanto ii bd facscanto ii 488nm 20mw 633nm 17mw. Standardization of 8color flow cytometry across different. Bd facscanto ii flow cytometer quick reference guide.
Bd facscanto ii features bd facscanto ii clinical cell. Bd facscanto ii canto 10color with canto clinical software. Bd facscanto clinical software overview features workflow sample data productivity, consistency, and reliability from dedicated clinical software the ivd cleared bd facscanto ii system includes software designed to address the needs of todays busy clinical laboratory. The bd facscanto ii system can optionally be used with the bd facs loader for automatic sample introduction, a standalone barcode reader for data input into bd facscanto clinical software, and with the bd facs sample prep assistant ii for automatic sample preparation of assays utilizing the lysenowash method. Bd facscanto ii 422 configuration automated sample presentation bd facs universal loader bd facsloader with updated motor, pneumatic actuation, and sliding access doors software bd facsuite clinical software with 4 and 6color assay modules facscanto clinical software with multitest 6color and multitest 4color assays included as panels. The bd facscanto comes with bd facscanto clinical software and bd facsdiva software. Bd facscanto ii canto 10color with canto clinical software february 2020 critical andor security patches.
M, and then stained using fitc annexin vpropidium iodide pi. The facscanto ii is a powerful machine and with its 3 laser 405, 488, 633 nm it. The bd facscanto ii is a powerful multicolour benchtop analyser offering easy to use, powerful and reliable performance. The bd facscanto ii system features many innovations.
The tbnk kits are capable of providing a complete immune panel t. The facscanto ii offers unbeatable consistency and optimal signal detection with increased resolution and sensitivity for multicolor assays. Clinical excellence support investors united states login. Use bd facscanto clinical software for performing cytometer quality. Services flowcytometry bd facscanto2 cedoc chronic. Bd bioscience facscanto ii flow cytometer marshall. Bd biosciences facscanto ii flow cytometer product list. For equivalency between methods, data n 362 were analyzed with deming regression for absolute count and percentage of lymphocytes.
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